Comparative Analysis of Universal Protein Extraction Methodologies for Screening of Lipase Activity from Agricultural Products

Protein extraction techniques are absolutely required for the research of biological catalysts. The present study compared four universal protein methodologies (ammonium sulfate precipitation, TCA/acetone and two commercial kits) to provide practical information on in order discover a novel lipase agricultural products. Yields from 24 domestic products their specific activities were evaluated with each other. precipitation showed relatively higher yield (on average, 3.41 ± 1.08 mg protein/0.1 g sample) crude extraction, whereas Pierce™ Plant Total Extraction Kit highest activity average both spectrophotometric (266.61 235.78 μU/mg protein) fluorometric (41.52 32.63 assays. Our results suggest that kits rapid soluble functional proteins would be better choice than conventional perform high-throughput screening enzyme plant sources. Finally, several such as cordyceps, pepper, bracken, hemp, all which exhibited an excellent activity, proposed promising candidates source lipases.